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Optimization of biosynthesis conditions and catalitic behavior evaluation of cellulase-free xylanase produced by a new Streptomyces sp. strain

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Author(s): GIGI COMAN | ADINA CONSTANTIN | GABRIELA ENACHE | LUMINITA GEORGESCU | GABRIELA BAHRIM

Journal: Annals of the University Dunarea de Jos of Galati. Fascicle VI : Food Technology
ISSN 1843-5157

Volume: 35;
Issue: 1;
Start page: 34;
Date: 2011;
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Keywords: cellulase-free xylanase | Streptomyces | submerge fermentation | thermostable enzyme

ABSTRACT
Cellulase-free xylanase by Streptomyces sp.P12-137 was obtained bycultivation on the wheat bran as the sole carbon source. The effect of carbon and nitrogen sources and a ratio of them on the cellulase-free xylanase production was investigated. The new isolate Streptomyces sp. strain was able to grow in submerged system and to produce an increased level of xylanase. Wheat bran induced xylanase biosynthesis yield at a high level (9.27 UA/ml). For economical reasons cultivation was achieved on a cheap fermentative medium represented by agro-industrial wastes. The optima of the pH and temperature of the crude xylanase activity were 5.5 and 70°C,respectively.

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