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Optimization of Cellulase Production by Trichoderma reesei HY07 Using Response Surface Methodology

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Author(s): Guowei Shu | Hui Yang | He Chen | Shikai Wang

Journal: Research Journal of Applied Sciences, Engineering and Technology
ISSN 2040-7459

Volume: 5;
Issue: 23;
Start page: 6438;
Date: 2013;
Original page

Keywords: Cellulose | carboxymethyl cellulose | FPA | response surface methodology | solid state fermentation

ABSTRACT
Response Surface Methodology (RSM) based on a three-level, three-variable Box and Behnken Factorial Design (BBFD) was used to evaluate the interactive effects of corn stalk: bran ratio, tween80 and temperature on the cellulase production by solid fermentation. The optimum conditions derived via RSM were: corn stalk: bran ratio 1.29:1, Tween80 11.05 &muL and temperature 31 °C for carboxymethyl cellulase (CMCase) and corn stalk: bran ratio 0.77:1, Tween80 12.54&muL and temperature 32 °C for Filter Paper Activity (FPA). The actual experimental yield was 406.42 U/g for CMCase and 93.62 U/g for FPA under optimum condition, which compared well to the maximum predicted value of 405.67 U/g and 91.29 U/g. The cellulase yield under optimal conditions was 1.45 fold for CMCase and 1.33 fold for FPA to the control.

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