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Optimization of Process Parameters for the Productionof Lipase in Solid State Fermentation by Yarrowia Lipolytica from Niger Seed Oil Cake (Guizotia Abyssinica)

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Author(s): Sarat Babu Imandi | Sita Kumari Karanam | Hanumantha Rao Garapati

Journal: Journal of Microbial & Biochemical Technology
ISSN 1948-5948

Volume: 02;
Issue: 01;
Start page: 028;
Date: 2010;
Original page

Keywords: Lipase | Niger seed oil cake | Optimization | Plackett– Burman design | Yarrowia lipolytic

ABSTRACT
The production of extra cellular lipase in Solid State Fermentation(SSF) using Yarrowia lipolytica NCIM 3589 withNiger seed oil cake (Guizotia abyssinica) has been made.Different parameters such as incubation time, inoculumlevel, initial moisture content, carbon level and nitrogen levelof the medium were optimized. Screening of various processvariables has been accomplished with the help ofPlackett–Burman design. The maximum lipase activity of26.42 units per gram of dry fermented substrate (U /gds)was observed with the substrate of Niger seed oil cake infour days of fermentation. STATISTICA 6.0 was used forimplementing Plackett–Burman design.
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