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Organic Acid Profiling Analysis in Culture Media of Lactic Acid Bacteria by Gas Chromatography-Mass Spectrometry

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Author(s): Jae Yeon Lee | Duc-Toan Nguyen | Young Shik Park | Kyo Yeol Hwang | Yong Seok Cho | Kyung-Don Kang | Jaehwan Yoon | Jundong Yu | Sung-Tae Yee | Young Hwan Ahn | Gwang Lee | Su-Il Seong | Man-Jeong Paik

Journal: Mass Spectrometry Letters
ISSN 2233-4203

Volume: 3;
Issue: 3;
Start page: 74;
Date: 2012;
Original page

Keywords: Organic acid | Profiling analysis | Lactobacillus pentosus K34 | Pediococcus lolli PL24 | GC-MS

ABSTRACT
Organic acid (OA) profiling analysis was performed in culture media from Lactobacillus pentosus K34 (L. pentosusK34) and Pediococcus lolli PL24 (P. lolli PL24) by gas chromatography-mass spectrometry (GC-MS) following methoxime/tert-butyldimethylsilyl derivatives. 12 OAs were positively identified in culture media. Most of OA levels from L. pentosus K34of hetero lactic fermentation were found to be higher when compared with those from P. lolli PL24 of homo lactic fermentation,which may explain different OA metabolism in each strain. In addition, the distorted dodecagonal star patterns were readily distinguishable,and the characteristics of each strain were well represented. The present study demonstrates that the OA metabolic profiling method by GC-MS combined with star pattern recognition is useful for the monitoring study of characteristic OA metabolism in various microorganisms.
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