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Overexpression of P53 in gastric carcinomas and its correlation with Lauren and Goseki classification

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Author(s): Luminita Ivan | Ovidiu Toma

Journal: Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara
ISSN 1582-3571

Volume: 10;
Issue: 2;
Date: 2009;
Original page

ABSTRACT
Despite their importance, prognostic factors in gastric cancer are not yet well known. The study included surgical specimens from 40 patients with gastric carcinoma. All specimens were fixed in 10% buffered neutral pH formaldehyde and paraffin embedded. Histological sections were stained using current techniques: haematoxylin-eosin, tricromic van Gieson, and Alcian blue. We used Laurén histological classification with two main types of gastric carcinoma: intestinal and diffuse and Goseki classification with four main types of gastric carcinoma. We assessed p53 immunoreactivity. (DO 7 Biogenex). Using Lauren classification, immunoreactivity was positive for p53 in 62.5% of the cases: 7 cases of well differentiated intestinal low index gastric carcinoma, 1 case of poorly differentiated intestinal low to moderate index gastric carcinoma, 15 cases of diffuse moderate to high index gastric carcinoma. Using Goseki classification, immunoreactivity was positive for p53 in 62.5% of the cases: 3 cases with low index and 1 case with moderate index in type I, 3 cases with low index in type II, 9 cases with low to high index in type III and 6 cases with low to high index in type IV. The positivity for p53 was expressed in 66% of the gastric carcinomas with a coloring intensity growth pattern from the well differentiated types to the poorly differentiated types. The percentage of tumors positive for p53 did not correlate with their differentiation degree.
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