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Oxidative stress disturbs energy metabolism of mitochondria in ethanol-induced gastric mucosa injury

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Author(s): Jin-Shui Pan, Shao-Zhen He, Hong-Zhi Xu, Xiao-Juan Zhan, Xiao-Ning Yang, Hong-Min Xiao, Hua-Xiu Shi, Jian-Lin Ren

Journal: World Journal of Gastroenterology
ISSN 1007-9327

Volume: 14;
Issue: 38;
Start page: 5857;
Date: 2008;
Original page

Keywords: Animal model | Ethanol | Gastric mucosa | Oxygen free radical | Energy metabolism

ABSTRACT
AIM: To study the role of mitochondrial energy disorder in the pathogenesis of ethanol-induced gastric mucosa injury.METHODS: Wistar rats were used in this study. A gastric mucosal injury model was established by giving the rats alcohol. Gross and microscopic appearance of gastric mucosa and ultrastructure of mitochondria were evaluated. Malondiadehyde (MDA) in gastric mucosa was measured with thiobarbituric acid. Expression of ATP synthase (ATPase) subunits 6 and 8 in mitochondrial DNA (mtDNA) was determined by reverse transcription polymerase chain reaction (RT-PCR).RESULTS: The gastric mucosal lesion index was correlated with the MDA content in gastric mucosa. As the concentration of ethanol was elevated and the exposure time to ethanol was extended, the content of MDA in gastric mucosa increased and the extent of damage aggravated. The ultrastructure of mitochondria was positively related to the ethanol concentration and exposure time. The expression of mtDNA ATPase subunits 6 and 8 mRNA declined with the increasing MDA content in gastric mucosa after gavage with ethanol.CONCLUSION: Ethanol-induced gastric mucosa injury is related to oxidative stress, which disturbs energy metabolism of mitochondria and plays a critical role in the pathogenesis of ethanol-induced gastric mucosa injury.

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