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Paraoxonase 1 gene (Gln<sup>192</sup>-Arg) polymorphism and the risk of coronary artery disease in type 2 diabetes mellitus

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Author(s): Rania Mohamed Azmy | Mostafa Mohamed Elnajjar | Mohamed Fahmy Elnoamany | Ashraf Abdelraouf Dawood

Journal: Advances in Molecular Imaging
ISSN 2161-6728

Volume: 02;
Issue: 01;
Start page: 29;
Date: 2011;
Original page

Keywords: Paraoxonase 1 | Coronary Artery Disease | Diabetes Mellitus.

ABSTRACT
Background: Paraoxonase 1 (PON1) is reported to have an antioxidant and cardioprotective properties. Recently, an association of glutamine (Gln) or (type A)/arginine (Arg) or (type B) polymorphism at posi-tion 192 of PON1 gene has been suggested with coro-nary artery disease (CAD) among patients with di-abetes mellitus (DM). However, conflicting results ha- ve also been reported. Objectives: To investigate the relationship between PON1 gene (Gln192-Arg) poly- morphism and the presence, extent and severity of CAD in type 2 DM. Methods: The study comprised 180 patients recruited from those undergoing corona- ry angiography for suspected CAD, who were divided according to the presence or absence of CAD and DM into 4 groups; Group I (n = 40 patients) nondiabetic subjects without CAD, Group II (n = 45 patients) di-abetic patients without CAD, Group III (n = 47 patients) non diabetic patients with CAD and Group IV (n = 48 patients) diabetic patients with CAD. PON1 (Gln192-Arg) genotype was assessed using polymerase chain reaction (PCR) followed by AlwI digestion. Results: The frequency of Gln allele (Type A) was significantly higher in group I and group II compared to group III and group IV (62.5%, 60% vs 38.3%, 31.25% respectively, p < 0.001) while the frequency of Arg allele (Type B + Type AB) was significantly higher in ischemic groups (III, IV) compared to non ischemic groups (I,II) (61.7%, 68.75% vs 37.5%, 40% respec-tively, p < 0.001). Patients with CAD and DM (group IV) have significantly higher severity score and vessel score than those with CAD only (group III) (9.7 ± 2.97, 2.44 ± 0.56 vs 6.99 ± 3.71, 1.67 ± 0.89 respectively, p < 0.001) Patients with vessel score 3 had significantly higher severity score and higher Arg allele frequency than patients with vessel score 2, the latter group had also significantly higher severity score and Arg allele frequency than patients with vessel score 1 (8.9 ± 2.79 vs 5.21 ± 2.13 and 80.49% vs 67.86%), (5.21 ± 2.13 vs 3.11 ± 0.89 and 67.86% vs 53.85%), p < 0.001 for all. In multivariate logistic regression analysis of different variables for prediction of CAD, age [OR 2.99, CI (1.11 - 10.5), P < 0.01], smoking [OR 4.13, CI (1.37 - 11.7), P < 0.001], low density lipoprotein (LDL) cholesterol > 100 mg/dL [OR 4.31, CI (1.25 - 12.5), P < 0.001], high density lipoprotein (HDL) cholesterol
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