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Pathogenicity, Antigenicity and Immunogenicity of Infectious Bursal Disease Virus (IBDV) Due to Propagation in Chicken Embryo Related Cell Line Using Serum-free Medium

Author(s): Tereza Cristina Cardoso | Maria Cec?lia B Teixeira | Natali Fachin | Daniela Pilz

Journal: Journal of Animal and Veterinary Advances
ISSN 1680-5593

Volume: 4;
Issue: 4;
Start page: 453;
Date: 2005;
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Keywords: IBDV | CER replication | Pathogenicity | Immunogenicity | Antigenicity | Serum free medium

Relative pathogenicity, antigenicity and immunogenicity of standard Lukert strain of IBDV were observed in Chicken Embryo Related (CER) cell maintained in Ex Cell 520 serum free medium following adaptation. It also used Lukert strain of IBDV propagated in Bursa of Fabricius (BF), chicken embryos and Chicken Embryo Fibroblast (CEF). Bursa derived-IBDV induced the most severe lesions in BF when compared with those propagated in CEF and CER cells, which produced mild lesions. Antibody induced by cell culture-derived IBDV neutralize bursa and embryo derived-IBDV less effectively, whereas antibody induced by CER-derived virus neutralize well CEF-derived IBDV. Moreover, after 14 days of IBDV infection, CEF and CER-derived IBDV produced same level of antibodies. A high frequency of virus re-isolation (obtained from bursae, spleen, thymus and bone marrow of infected birds) was observed by bursa and embryo derived-IBDV at all p.i. However the viral RNA detected by RT-PCR showed positive results for all negative ones in virus re-isolation. These results document that the relative pathogenicity, antigenicity and immunogenicity is not reduce significantly following IBDV adaptation in CER cells, using serum free medium, compared with those obtained by CEF-derived IBDV.

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