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Pathogenicity of human high pathogenic H5N1 virus with different plaque property

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Author(s): Yong-qiang LI | Jing LI | Yi HU | Wei SUN | Guo-hui CHANG | Yin-hui YANG | Xiao-ping KANG | Xiao-yan WU | Qing-yu ZHU

Journal: Medical Journal of Chinese People's Liberation Army
ISSN 0577-7402

Volume: 36;
Issue: 6;
Start page: 582;
Date: 2011;
Original page

Keywords: influenza A virus | H5N1 subtype´╝Ť virulence

ABSTRACT
Objective To determine the pathogenicity of human high pathogenic H5N1 virus with different plaque property,and provide a new train of thought and basis for research on crossing species-genus transmission of avian influenza virus.Methods Variants with different plaque property(larger-and smaller-plaque variant) were isolated from A/Beijing/01/03(H5N1)(BJ01) by the plaque purification.Mice were inoculated intranasally(i.n.) with each kind of isolated variant and monitored for 14 days to record and calculate the body weight change and the survival rate.The difference in pathogenicity of isolated variants was estimated with proper principle of statistics.Results The plaques of original strain of BJ01 virus were heterogeneous with various size and shape.Larger-plaque variants(L1 and L2) and smaller-plaque variants(S1,S2 and S3) were isolated separately.The pathogenicity was higher in S1 and S2 variants than in S3,L1 and L2 variants,and that of L2 variant was the lowest.Variants forming different plaques were heterogeneous in pathogenicity,and those forming same plaques were also heterogeneous in pathogenicity.Conclusion There is no linear correlation between the pathogenicity of variants and the size of plaque they formed.The variants with different pathogenicity could be isolated from the same H5N1 virus strain by plaque purification.These results would provide a basis for vaccine development and the studies on molecular mechanism of H5N1 virus.

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