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PCR detection of white spot syndrome virus (WSSV) from farmed Pacific white shrimp (Litopenaeus vannamei) in selected sites of the Philippines

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Author(s): Benedict A. Maralit | Christopher M. A. Caipang | Mudjekeewis D. Santos | Mary Beth B. Maningas

Journal: Aquaculture, Aquarium, Conservation & Legislation
ISSN 1844-8143

Volume: 4;
Issue: 4;
Start page: 474;
Date: 2011;
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Keywords: WSV | Philippines | shrimp | Litopenaeus vannamei

ABSTRACT
Great losses caused by white spot syndrome virus (WSSV) in shrimp culture have beenattributed to poor screening procedures in farms and the lack of sufficient access to specific pathogenfree brood stock. Thus, early detection of the virus is considered the best option for shrimp farmers. Thestudy, thus, assessed viral incidence in the Philippines and partially sequenced and characterized thePhilippine WSSV isolate with regards to other isolates in GenBank. Developed primers for PCR can detecttarget genes from 0.4 pg of DNA extract from shrimp samples. PCR detection revealed that 6.67 %(1/15) of market samples from Zambales are infected with WSSV. Shrimp samples from a local shop anda public market in General Santos City showed 46.67% (7/15) and 20% (3/15) WSSV-positive samplesrespectively. Shrimp sources from Capiz and Batangas, however, showed negative detection for WSV. Nosignificant difference in the number of infected samples from the sampling sites was found. Combineddetections reveal that the Philippines has a low infection rate of 14.67%. The study has partiallysequenced and characterized Philippine isolate. During the sampling period, most shrimps in GeneralSantos City were WSSV-positive by PCR detection.
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