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PCR Primer Design: DREB Genes

Author(s): Neha Garg | Sachin Pundhir | Anil Prakash | Anil Kumar

Journal: Journal of Computer Science & Systems Biology
ISSN 0974-7230

Volume: 01;
Issue: 01;
Start page: 021;
Date: 2008;
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The polymerase chain reaction (PCR) is an enzymatic reaction which follows simple, predictable and well understood principles. Selective amplification of nucleic acid molecules, that are initially present in minute quantities, provides a powerful tool for analyzing nucleic acids. In this context, efficiency and sensitivity of the PCR largely depends on the efficiency of the primers that are employed for the amplification of a concerned gene. Environmental adversities like drought resulting in scarcity of water have detrimental effects on crop yields worldwide. Sustainable agricultural and food productivity requires development of stress resistant plant species like drought resistant crops that can with stand and flourish in scanty water level environments. A key to underlying such attempts is the molecular understanding of the discrete stress processes that are interwoven at multiple levels. In this review, we discuss about some of the contemporary developments in the area of stress resistance by plants along with the various approaches for the PCR primer designing of two key genes involvedin drought resistance, DREB1A and DREB2A.
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