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PCR Screening of Barley Genotypes in Relation to Cold Hardiness

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Author(s): M. Sinan Taspinar | Sevim Sagsoz | Guleray Agar | Metin Tosun | Ilknur Akgun

Journal: International Journal of Botany
ISSN 1811-9700

Volume: 4;
Issue: 3;
Start page: 315;
Date: 2008;
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Keywords: Barley | cold-induced genes | field survival index | environmental stress

ABSTRACT
Several molecular methods were tested to distinguish 24 barley genotypes based on cold hardiness. We used a convenient, efficient and reliable PCR based method for barley to determine the cold tolerant and sensitive genotypes. Cold hardiness was strongly correlated with the existence of cold-induced genes. Significant differences were found based on Field Survival Index (FSI) of genotypes. Based on combination of FSI values and PCR detection pattern of cold-induced genes, PCR analysis can be used in plant breeding programs in order to shorten selection period at environmental stress studies and get exact results.
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