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Pharmacokinetic Evaluation of Metolazone Tablets using Healthy Human Volunteers

Author(s): Basvan Babu | Selvadurai Muralidharan | Subramaniya Nainar Meyyanathan | Bhojraj Suresh

Journal: Journal of Bioequivalence & Bioavailability
ISSN 0975-0851

Volume: 02;
Issue: 01;
Start page: 015;
Date: 2010;
Original page

Keywords: Metolazone | Bioavailability studies | Pharmacokinetic evaluvation

A sensitive and reproducible high performance liquid chromatography (HPLC) method has been developed and validated for the quantification of metolazone in human plasma, after solid phase extraction (SPE). A Good resolution was achieved on a reverse-phase LichroCART Purospher® C18 column using the mobile phase acetonitrile–0.5% triethylamine (35:65) in isocratic elution with a total run time of 15 min. The analyte, metolazone, was detected by using high performance liquid chromatography with the support of photo diode array detector. Limit of detection and Lower limit of quantification was found to be 1 and 2.5 ng/mL. The present method was successfully applied in the pharmacokinetic study of metolazone in human plasma.
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