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Phenotypic detection of inducible Clindamycin resistance among Staphylococcus species

Author(s): Reshmi C Roy | Shitanshu Kaundal | Sulekha Nautiyal, | Shalabh Jauhari | Bhim S Mahawal | Ankit Khanduri

Journal: International Journal of Advanced Research
ISSN 2320-5407

Volume: 1;
Issue: 8;
Start page: 12;
Date: 2013;
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Keywords: Clindamycin | Erythromycin resistance | Staphylococcus sp. | D-test | iMLSB | cMLSB.

Background: Clindamycin is commonly used for the treatment of Staphylococcal infection for its excellent pharmacokinetic properties. The Staphylococcus species that are resistant to erythromycin and susceptible to clindamycin may show inducible resistance due to expression of erm gene resulting in treatment failure. The study was undertaken to detect the inducible clindamycin resistance by in vitro D-test on the Staphylococcal isolates obtained from samples of indoor and outdoor patients, attending Shri Mahant Indiresh Hospital, Dehradun. Materials and methods: A total of 168 Staphylococcus species were isolated from different clinical samples. Erythromycin resistant strains were subjected to ā€˜Dā€™ test for the detection of inducible clindamycin resistance as per CLSI guidelines. Results were analyzed statistically. Result: Four different phenotypes (iMLSB, cMLSB, MS, sensitive both erythromycin and clindamycin) were observed among MRSA, MSSA and coagulase negative S. aureus. 42.8% methicillin resistant S.aureus and 16.4% methicillin sensitive S.aureus isolates showed inducible clindamycin resistant phenotype. MRSA isolates showed significantly higher iMLSB phenotype (P= 0.0001). Conclusion: This study strongly proposes routine in vitro D-test of Staphylococcus species, which in turn will help in taking appropriate therapeutic decisions.
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