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Phosphorus and Nitrate Removal by Selected Wastewater Protozoa Isolates

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Author(s): O.B. Akpor | M.N.B. Momba | J. Okonkwo

Journal: Pakistan Journal of Biological Sciences
ISSN 1028-8880

Volume: 10;
Issue: 22;
Start page: 4008;
Date: 2007;
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Keywords: protozoa | removal | nitrate | Phosphorus | wastewater

ABSTRACT
In the present study, the efficiency of ten selected wastewater protozoa in the removal of phosphorus and nitrogen and their effects on other physico-chemical parameters was investigated. Protozoa were isolated from the aerobic zone of Daasport wastewater treatment plant, in Pretoria, South Africa, using the modified Chalkey’s medium. The isolates were screened for nutrient removal ability in shake flasks, at laboratory temperatures for 96 h. During each sampling, aliquot samples were collected for the analysis of phosphorus, nitrate-nitrogen, Chemical Oxygen Demand (COD), Dissolved Oxygen (DO), pH, of the mixed liquor including the growth rate of the isolates using standard methods. The results indicated a steady increase in growth rate of the isolates and all the isolates used in this study showed some measure of phosphorus and nitrate removal ability. In the presence of the test protozoa isolates, an increase in COD and a decrease in DO were noted in the mixed liquor inoculated with the organisms, while the pH only increases slightly. This study revealed a significant correlation between growth rate and nutrient removal (R = -0.806, p
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