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Phytochemical analysis and Antimicrobial activities of different plant parts of Vitex negundo L.

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Author(s): Sujogya Kumar Panda1*, Laxmipriya Padhi2, Gargee Mohanty1, Gunanidhi Sahoo2, Sushil Kumar Dutta2

Journal: Journal of Pharmacy Research
ISSN 0974-6943

Volume: 4;
Issue: 9;
Start page: 3184;
Date: 2011;
Original page

Keywords: Similipal Biosphere Reserve | phytochemicals | MIC | MBC | Vitex negundo

ABSTRACT
In the present work leaf and bark extracts of V. negundo were screened for antibacterial and anticandidal property. Qualitative phytochemical analysis, agarcup method, micro-dilution method for MIC, TLC bioautography were used as prescreening methods to study antimicrobial properties against eleven Gramnegative,five Gram-positive pathogenic bacteria and four Candida species. One way ANOVA was carried out to find out the existence of significant differencesin the total activity (TA) and zone of inhibitions between each solvent extracts. Furthermore, Duncans post hoc test was carried out to determine the differencein the TA and zone of inhibitions. All five extracts (Petroleum ether, chloroform, ethanol, methanol and water) exhibited significant activity with zone ofinhibitions 9.6-15.6 mm for bark and 9.3-14.3 for leaf. Ethanol extracts were best for both bark and leaf with MIC (313 to 2500 μg/ml) for leaf and (313 to5000 μg/ml) for bark. Considering the quantity extracted from bark powder, the total activity was highest for ethanol and lowest for chloroform. TLC bioautographyshowed zone of inhibition (>20 mm) with Rf value of 0.72 for ethanol extract against S. flexneri. Preliminary phytochemical analysis exhibitedpresence of carbohydrates, triterpenoids, tannin and phenolic compounds, glycosides, steroid and sterols and flavonoids in different extracts.
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