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Phytochemical evaluation and polyphenolic antioxidant activity of methanolic root extract of Desmostachya bipinnata(Poaceae)

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Author(s): Kalpana. P. Rahate *1, 2A Rajasekaran, 3Manju Prathap

Journal: Journal of Pharmacy Research
ISSN 0974-6943

Volume: 4;
Issue: 9;
Start page: 3017;
Date: 2011;
Original page

Keywords: Desmostachya bipinnata | flavonoids | tannins | superoxide | reducing power | TBARS | ABTS

ABSTRACT
Our study was aimed at screening of antioxidant activity and the (components) present in the plant which is responsible for the antioxidant activity. Methanolicextract of roots of Desmostachya bipinnata was evaluated for phytochemical parameters. The total phenolic content was determined using folin-ciocalteu assay.The content of total flavonoids was also measured spectrophotometrically by using aluminium chloride colorimetric assay and the content of tannins wasdetermined by Folin-Denis method. The scavenging activity on superoxide, ABTS+ and the reducing power was determined. Inhibition of lipid peroxidation wasstudied by thiobarbituric acid reactive substances (TBARS) method on isolated goat liver tissues. The plant showed moderate anti-lipid peroxidation againstthiobarbituric acid (69.422±0.7148%). The extract exhibited scavenging activity towards superoxide and ABTS+ radicals (IC50 64.66 and 93.64 μg/ml) due to thepresence of relatively high total phenol and flavonoids contents (25.62 ± 0.10 and 150.88±12.61μg/ml respectively). Our findings suggest that Desmostachyabipinnata. is endowed with antioxidant phytochemicals and could serve as a base for future drugs.
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