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PHYTOCHEMICAL INVESTIGATION AND IN VITRO ANTIOXIDANT ACTIVITY OF SOME MEDICINALY IMPORTANT PLANTS OF UTTARAKHAND

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Author(s): Dua Virender Kumar | Mathur Abhishek | Verma Satish Kumar | Singh Santosh Kumar | Prasad GBKS

Journal: International Research Journal of Pharmacy
ISSN 2230-8407

Volume: 2;
Issue: 6;
Start page: 116;
Date: 2011;
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Keywords: Microwave Solvent Extraction (MSE) | Antioxidant activity | DPPH assay | Superoxide Anion Radical Scavenging activity | Total Phenolic Content (TPC).

ABSTRACT
Antioxidants are the substances, compounds or nutrients in our foods which can prevent or slow oxidative damage to our bodies. These agents are able to remove the deleterious effects of free radicals within our body. Solvent extraction methods are widely used for extracting antioxidant in foods and other sources. In this study, Microwave Solvent Extraction (MSE) methods were utilized to determine the content of antioxidants in Riccinus communis (root), Aloe vera (leaves), Crateva nurvula (bark), Swertia chirayta (whole plant) and Bacopa monnieri (whole plant) extracts. MSE was performed at 80ÂșC employing four solvents, methanol, aqueous, hexane and petroleum ether. The in vitro antioxidant activity was determined by DPPH (2, 2-Diphenyl-1-picrylhydrazyl) assay and Superoxide Anion Radical Scavenging activity. The yield of antioxidants extracted using the MSE was achieved using methanol as the solvent, followed by aqueous extracts in all the plants in comparison to Riccinus communis. Antioxidant activities in hexane and petroleum ether extracts of Riccinus communis were efficient in comparison to that of methanol and aqueous extracts of other plants. The antioxidant capacities were found to be correlated with the Total Phenolic Content (TPC). The phytochemical active constituents in the potent extracts were determined by conventional methods. These studies thus lead to the isolation and characterization of some active components responsible for antioxidant activity.
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