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Phytochemical studies and GC/MS analysis on the isolated essential oil from the leaves of Piper betle L.var.sirugamani1(SGM1)

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Author(s): K Periyanayagam1*,M Mubeen1, M Mohamed Saleem2 and S Sathik Basha3

Journal: Journal of Pharmacy Research
ISSN 0974-6943

Volume: 4;
Issue: 7;
Start page: 2411;
Date: 2011;
Original page

Keywords: Piper betle | Phytochemical studies | Fluorescence analysis | Essential oil | Germacrene-D | GC/MS analysis.

ABSTRACT
Piper betle is a deep green heart shaped , popularly known as paan in India have many uses in Chinese and Indian ethnomedicine. Piper betle L. var. Sirugamani1 (SGM1) (Piperaceae) was selected as it is widely cultivated and used. Phytochemical studies, fluorescence analysis on the dried powdered leaves and gas chromatomatography- mass spectrometry (GC-MS) analysis on the essential oil from the fresh leaves were undertaken. Preliminary phytochemical screening onthe leaves revealed the presence of carbohydrate, phytosterols, saponins, tannins, proteins and free amino acids, mucilage, terpenoids and flavonoids. Considerable colour variations were observed in the fluorescence analysis. 0.8% wt/v of essential oil was obtained. GC/MS fingerprint showed the presence of 50 compounds inthe oil. The major compounds were Germacrene-D(16.07%) followed by Lepidozene(14.99), b-caryophyllene (9.86%), 1,3,4 –Eugenol (7.17%), ß-Elemene, ?- Murrolene (3.18%), a-Selinenol (3.07), b-Cadinene (2.82%) and Cineole (2.80%). Some variations were observed in the composition and percentage of constituents in the essential oil when compared to the previous studies. This might be due to the climate, soil and other conditions. It is concluded that the polyphenols present in the essential oil may provide a good candidate of various effective pharmaceutical formulations.
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