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Plasmid mediated drug resistance and anti-MRSA activity of Pongamia glabra and Ocimum basilicum

Author(s): Ramesh K, Gifson Gnanadhas Y, Karthick N, Umamaheswari S *

Journal: Journal of Pharmacy Research
ISSN 0974-6943

Volume: 4;
Issue: 8;
Start page: 2466;
Date: 2011;
Original page

Keywords: MRSA | plasmid | Antimicrobial activity | Pongamia glabra and Ocimum basilicum.

A total of 60 wound specimens were processed for isolation of Methicillin Resistant Staphylococcus aureus (MRSA) by selective medium and four isolates were confirmed as MRSA. Plasmid mediated drug resistance of the isolates was confirmed by growth of plasmid cured organisms on medium containing methicillin. Anti- MRSA activity of Pongamia glabra and Ocimum basilicum plant extracts (aqueous, methanolic, ethanolic) in different concentrations were tested by agar well diffusion method. The plant extracts were subjected to various preliminary phytochemical analysis to determine its phytochemical constituents. Methanolic and ethanolic extracts of both the plants exhibited more activity against the tested bacteria (MRSA) as compared to aqueous extract. The study also revealed that,Ocimum basilicum is found to possess more potent anti-MRSA activity rather than Pongamia glabra.
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