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Polyunsaturated Fatty Acids and Modulation of Cholesterol Homeostasis in THP-1 Macrophage-Derived Foam Cells

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Author(s): Masoud Salehipour | Ebrahim Javadi | Javad Zavvar Reza | Mahmoud Doosti | Shahla Rezaei | Malihe Paknejad | Naser Nejadi | Mansour Heidari

Journal: International Journal of Molecular Sciences
ISSN 1422-0067

Volume: 11;
Issue: 11;
Start page: 4660;
Date: 2010;
Original page

Keywords: THP-1 | conjugated linoleic acid | α-linlenic acid | eicosapentaenoic acid | liver X receptor α | ATP-binding cassette A1

ABSTRACT
Transformation of macrophages to foam cells is determined by the rates of cholesterol uptake and efflux. This study uses a real time RT-PCR technique to investigate the role of conjugated linoleic acid (CLA), α-linolenic acid (ALA) and eicosapentaenoic acid (EPA) in the regulation of the ATP-binding cassette A1 (ABCA1) and liver X receptor α (LXR) genes, which are involved in cholesterol homeostasis. Accordingly, these fatty acids significantly reduced the total, free and esterified cholesterols within the foam cells. While the expression of the ABCA1 and LXRα genes was increased in the presence of the pharmacological LXRα ligand, T0901317, their mRNA expression was not significantly affected by CLA, ALA and EPA. These results suggest that although polyunsaturated fatty acids have an effect on cholesterol homeostasis, they cannot change the expression of the ABCA1 and LXRα genes. Alternatively, several other genes and proteins may be involved.
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