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Porcine RPL28 Gene, Differentially Expressed in the Backfat Tissue Between Meishan, Large White Pigs and Their F1 Hybrids

Author(s): Z.Q. Ren | Y.Z. Xiong | W.J. Wu | C.Y. Deng | S.W. Jiang

Journal: Journal of Animal and Veterinary Advances
ISSN 1680-5593

Volume: 11;
Issue: 8;
Start page: 1237;
Date: 2012;
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Keywords: RPL28 | differential expression | pig | heterosis | backfat | China

The mRNA differential display technique was performed to investigate the differences in gene expression in the backfat tissues between Meishan, Large White pigs and their F1 hybrids. One novel gene that was differentially expressed was identified through both semi-quantitative Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and Quantitative reverse transcriptase Polymerase Chain Reaction (Q-PCR) and it was highly expressed in F1 hybirds, Meishan x Large White and Large White x Meishan compared to their parents. The full-length cDNA was then cloned using the Rapid Amplification of cDNA Ends (RACE) Method. The nucleotide sequence of the gene is not homologous to any of the known porcine genes. The sequence prediction analysis revealed that the open reading frame of this gene encods a protein of 137 amino acids has high homology with the RPL 28 of seven species-cattle (97%), human (97%), mouse (95%) and rat (97%) so that it can be defined as the porcine RPL28 gene. Tissue expression profile analysis indicated that the swine RPL28 gene is differentially expressed in detected tissues. The experiment suggested that the swine RPL28 gene might play role in the formation of heterosis.
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