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A Potentiometric Indirect Uric Acid Sensor Based on ZnO Nanoflakes and Immobilized Uricase

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Author(s): Syed M. Usman Ali | Zafar Hussain Ibupoto | Muhammad Kashif | Uda Hashim | Magnus Willander

Journal: Sensors
ISSN 1424-8220

Volume: 12;
Issue: 3;
Start page: 2787;
Date: 2012;
Original page

Keywords: ZnO nanoflakes (ZnO-NFs) | potentiometric nanosensor | uricase | NafionĀ® membrane

ABSTRACT
In the present work zinc oxide nanoflakes (ZnO-NF) structures with a wall thickness around 50 to 100 nm were synthesized on a gold coated glass substrate using a low temperature hydrothermal method. The enzyme uricase was electrostatically immobilized in conjunction with Nafion membrane on the surface of well oriented ZnO-NFs, resulting in a sensitive, selective, stable and reproducible uric acid sensor. The electrochemical response of the ZnO-NF-based sensor vs. a Ag/AgCl reference electrode was found to be linear over a relatively wide logarithmic concentration range (500 nM to 1.5 mM). In addition, the ZnO-NF structures demonstrate vast surface area that allow high enzyme loading which results provided a higher sensitivity. The proposed ZnO-NF array-based sensor exhibited a high sensitivity of ~66 mV/ decade in test electrolyte solutions of uric acid, with fast response time. The sensor response was unaffected by normal concentrations of common interferents such as ascorbic acid, glucose, and urea.
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