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Preliminary Analysis of gra SR for Vancomycin Resistance in Staphylococcus aureus Local Isolates

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Author(s): Dlnya A. Mohamad

Journal: Asian Journal of Medical Sciences
ISSN 2040-8765

Volume: 4;
Issue: 2;
Start page: 85;
Date: 2012;
Original page

Keywords: Burn | mrsa | two component signal | vrsa

ABSTRACT
Methicillin-resistant Staphylococcus aureus has become a frequent cause of nosocomial infection. Extensive burn injuries are particularly susceptible to infection. Extended hospitalization and antibiotic therapy have been identified as additional risk factors for MRSA carriage and infection, since prolonged use of antibiotics change the bacterial response to the surrounding medium and increase the mutation rate especially to the targeted site and also to the bacterial signal transduction system leading to the occurrence of the new strain showing various resistant mechanisms to the old as well as new antibiotic such as vancomysin. The aim of this study was to analyze response regulator graR gene to determine if the mutation in the signal transduction system lead to the variable susceptibility to vancomycin. Ten VRSA isolated from burned patients in the Burns Unit at sulaimani emergency hospital in 2008. All the isolates were $- lactamase producers. The PCR based identification technique used in this study proved to be rapid and accurate for the detection the genes regulate the vancomysin availability (sensor kinase and response regulator gra SR). Comparison of gra R DNA sequence and translated nucleotide sequence with N315 DNA and translated nucleotide sequences showed substitution of T553 instead of C, C554 instead of G, G556 instead of T, C565 instead of T and G 568 instead of A in N315 DNA sequence. This nucleotide substitution result in alteration of Gln548 instead of Asp and Asn717 instead of Ser amino acid in N315. No mutation was detected in graS gene.
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