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Preliminary Identification of Cultured Human Trophoblasts

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Author(s): Chunmei Wang | Xiaofeng Huang | Zhen Li | Dezhong Xu | Ke Men | Anhui Wang | Jiaji Yang | Hua Yu | Dan Chen | Xiaojun Huang | Shuang Wang

Journal: Research Journal of Biological Sciences
ISSN 1815-8846

Volume: 1;
Issue: 1-4;
Start page: 63;
Date: 2006;
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Keywords: Trophoblast | microscopy/electron | scanning | transmission | Laser Scanning Confocal Microscopy (LSCM)

ABSTRACT
To identify the characteristics of the cultured cells isolated from human villous. The purified and subcultured cells were derived from human placentae villous. The cellular morphology was observed under inverted phase contrast microscope. The nucleus and cytoplasm were demonstrated by Haematoxylin and Eosin (HE) staining. The superficial structure was observed under Scanning Electron Microscope (SEM) and the intracellular ultrastructures were revealed by transmission electron microscopy (TEM). The Immunofluorescent Histochemistry (IH) combined with Laser Scanning Confocal Microscopy (LSCM) was used to examine the expression of cytokeratin 18 (CK18), vimentin (Vim), -human Chorionic Gonadotropin ( -hCG) and human Placental Lactogen (hPL). The cultured cells appeared as patchy spreading and irregular polygonal with big oviform nucleus and transparent cytoplasm. SEM observation showed that abundant microvilli existed on the cell surfaces. The typical desmosomes between cells were observed with TEM. The numerous microvilli were also found on the surface of the cells. The abundant mitochondria, glycogen, developed Golgi complexes, a few of endoplasmic reticulum and many lipid droplets appeared in cytoplasm. Cytokeratin 18 expressed in all cells and vimentin was expressed in part of the cells, hCG and hPL immunoreactive positive substances were distributed in cytoplasm.
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