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Preparation and Characterisation of Collagen from Freshwater Fish Scales

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Author(s): Fengxiang Zhang | Anning Wang | Zhihua Li | Shengwen He | Lijun Shao

Journal: Food and Nutrition Sciences
ISSN 2157-944X

Volume: 02;
Issue: 08;
Start page: 818;
Date: 2011;
Original page

Keywords: Carp Fish Scale | ASC (Acid-Solubilized Collagens) | PSC (pepsin -solubilized collagens) | Type I Collagen

ABSTRACT
Acid-soluble collagen (ASC) and pepsin-solubilized collagen (PSC) were prepared from the waste freshwater carp fish scales. The results of SDS-PAGE showed that purified collagens were composed of at least two different chains which were in accordance with the type I collagen with α chain composition of (α1)2α2. Compared with the carp fish ordinary muscle type I collagen , porcine dermis type I collagen and other seawater fish collagens, freshwater carp fish scales collagen contained relative high half-cystine (Cys-s), but lower denaturation temperature(Td) than the porcine dermis type I collagen. These collagens had evident absorption at 230 nm by UV-Vis spectra. The spectrum X-ray diffraction showed that the collagen remained single-helix and tri-helix configuration with the minimum values of the repeat spacings (d) of about 4.48 Å and 11.87 Å. Therefore, to make more effective use of limited-resources, carp fish scales can be a potential resource for the extraction of type I collagen or gelatin.
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