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Preparation of Novel Poly(hydroxyethyl methacrylate-co-glycidyl methacrylate)-Grafted Core-Shell Magnetic Chitosan Microspheres and Immobilization of Lactase

Author(s): Wei Zhao | Rui-Jin Yang | Ting-Ting Qian | Xiao Hua | Wen-Bin Zhang | Wendy Katiyo

Journal: International Journal of Molecular Sciences
ISSN 1422-0067

Volume: 14;
Issue: 6;
Start page: 12073;
Date: 2013;
Original page

Keywords: magnetic chitosan microspheres | reversed-phase suspension polymerization | immobilization | lactase

Poly(hydroxyethyl methacrylate-co-glycidyl methacrylate)-grafted magnetic chitosan microspheres (HG-MCM) were prepared using reversed-phase suspension polymerization method. The HG-MCM presented a core-shell structure and regular spherical shape with poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) grafted onto the chitosan layer coating the Fe3O4 cores. The average diameter of the magnetic microspheres was 10.67 μm, within a narrow size distribution of 6.6–17.4 μm. The saturation magnetization and retentivity of the magnetic microspheres were 7.0033 emu/g and 0.6273 emu/g, respectively. The application of HG-MCM in immobilization of lactase showed that the immobilized enzyme presented higher storage, pH and thermal stability compared to the free enzyme. This indicates that HG-MCM have potential applications in bio-macromolecule immobilization.
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