Academic Journals Database
Disseminating quality controlled scientific knowledge

Prevalence of tst, entC, entA and entA/C genes in staphylococcus aureus strains isolated from different foods

ADD TO MY LIST
 
Author(s): Eshraghi S | Salehipour Z | Pourmand MR | Rahimi Forushani A | Zahraei Salehi MT | Agha Amiri S | Bakhtyari R | Abedi Mohtasab TP | Mardani N | Seyed Amiri S | Soltan Dallal MM

Journal: Tehran University Medical Journal
ISSN 1683-1764

Volume: 67;
Issue: 7;
Start page: 470;
Date: 2009;
Original page

Keywords: Staphylococcus aureus | toxic shock syndrome toxin-1 | enterotoxin A | enterotoxin C

ABSTRACT
"nBackground: Staphylococcus aureus is a major foodborne pathogen throughout the world. Enterotoxins and toxic shock syndrome toxin-1 are important virulence factors and as pyrogenic toxin superantigens have profound effects on the ir host. Thus circulation of TSST1 producing S.aureus among people and food chain is a worrying issue. The present paper was conducted to study Prevalence of tst, entC, entA and entA/C genes in staphylococcus aureus strains isolated from different foods. "n"nMethods: Over 1040 food samples have been analyzed differentially according to Iran national standard (number= 1194) for S.aureus identification. After DNA extraction, PCR reactions were carried out by reference strain as positive control, adequate primers. "n"nResults: At present study, prevalence of foodstuffs contaminated by S.aureus isolates was about 9.5% (100 strains). Of 25% of isolates producing entC, 28% (seven strains) had tst gene at the same time and of 8% of isolates producing entA, 12.5% (one strain) were positive for tst genes simultaneously. Altogether of 9% isolates producing combination of entC and entA, 44.4% (four strains) were also producer of tst gene. "n"nConclusion: Prevalence of TSST1 producing strains in combination with enterotoxin genes is considerable especially with entC and A plus C. On the other hand, circulation of these isolates in humans, animals, foods and environment has hazardous effect for general public health.
Why do you need a reservation system?      Affiliate Program