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Production of pectinase by Aspergillus niger cultured in solid state media.

Author(s): Nazneen Akhter 1, M. Alam Morshed 1,3* , Azim Uddin 3 , Feroza Begum 2 , Tipu Sultan 1 , Abul Kalam Azad 1

Journal: International Journal of Biosciences
ISSN 2220-6655

Volume: 1;
Issue: 1;
Start page: 33;
Date: 2011;
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Keywords: Pectinase | Pectin | Aspergillus sp. Solid state fermentation.

Solid state fermentation was carried out with 7 fungal strains, obtained from different sources. Among 7 isolates Aspergillus niger, IM-6 was found as effective pectinase producer. Maximum enzymatic activity (142.44U/gm) was observed after 7 days incubation at 40˚C temperature in 750 ml conical flask. In this study 1.69% (NH4)2SO4 was used as nitrogen source, although peptone as a nitrogen source showed better result but use of peptone was not cost effective. As a substrate, wheat bran and potato starch showed good result (85.54U/gm) in solid state culture. Addition of 9.68% pectin was found to increase the enzyme production as 116.57U/gm. Pectinase production was optimum in 60% moisture (98.34U/gm). Aeration showed positive effects on pectinase production (136.86U/gm) at 750 ml flask than 1000 ml flask. Thus the wild strain Aspergillus niger IM-6 has outstanding pectinase producing capability at 40◦C in 60% initial moisture content for 7 days of incubation insolid state fermentation.

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