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Programmed cell death induced by Salt stress in wheat cell suspension

Author(s): Ali Rezaei | Mohammadreza Amirjani | Majid Mahdiyeh

Journal: International Journal of Forest, Soil and Erosion
ISSN 2251-6387

Volume: 3;
Issue: 1;
Start page: 35;
Date: 2013;
Original page

Keywords: Programmed cell death | Salt stress | Wheat | DNA fragmentation

Programmed cell death (PCD) plays an essential role in many aspects of plant development and growth. Also it a key element in response to stresses. In the present study, we investigated role salt stress in induced programmed cell death or apoptosis wheat (Triticum aestivum L.) suspension cell culture. Wheat suspension cells were exposed to 500 Mm NaCl and cell death was determined using several apoptosis assay. We observed morphological and biochemical apoptosis features such as nuclear condensation, DNA laddering, transferase mediated dUTP nick end labeling. Positive TUNEL and reduction cell viability results were obtained treatment wheat suspension cell. The results indicate that 500 mM NaCl can induced pcd in suspension cell.
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