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Protective effect of prednisolone on ischemia-induced liver injury in rats

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Author(s): Meng Wang, Feng Shen, Le-Hua Shi, Tao Xi, Xi-Feng Li, Xu Chen, Meng-Chao Wu

Journal: World Journal of Gastroenterology
ISSN 1007-9327

Volume: 14;
Issue: 27;
Start page: 4332;
Date: 2008;
Original page

Keywords: Ischemia-reperfusion | Prednisolone | Cell membrane bleb | Calpain μ | Talin

ABSTRACT
AIM: To investigate the effects of prednisolone on cell membrane bleb formation, calpain μ activation and talin degradation during hepatic ischemia-reperfusion injury in rats.METHODS: The hilar area of the left lateral and median lobes of rat liver (68%) was clamped for 60 min and followed by 120 min reperfusion. Prednisolone was administered at 1.0, 3.0, or 10 mg/kg at 30 min before ischemia. In addition to biochemical and microscopic analyses, activation of calpain μ was determined using specific antibodies against the intermediate (activated) form of calpain μ. Degradation of talin was also studied by Western blotting.RESULTS: In the control and prednisolone (1.0 mg/kg) groups, serum aspartate transaminase (AST) and alanine transaminase (ALT) level were elevated, and cell membrane bleb formation was observed after 120 min of reperfusion. Moreover, calpain μ activation and talin degradation were detected. Infusion of prednisolone at 3.0 or 10 mg/kg significantly suppressed serum AST and ALT, and prevented cell membrane bleb formation. At 10 mg/kg, prednisolone markedly suppressed calpain μ activation and talin degradation.CONCLUSION: Prednisolone can suppress ischemia-reperfusion injury of the rat liver. Its cytoprotective effect is closely associated with the suppression of calpain μ activation and talin degradation.
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