Academic Journals Database
Disseminating quality controlled scientific knowledge

Protective effects of curcumin on retinal Müller cell in early diabetic rats

ADD TO MY LIST
 
Author(s): Zhong-Fu Zuo | Qiang Zhang | Xue-Zheng Liu

Journal: International Journal of Ophthalmology
ISSN 2222-3959

Volume: 6;
Issue: 4;
Start page: 422;
Date: 2013;
VIEW PDF   PDF DOWNLOAD PDF   Download PDF Original page

Keywords: diabetic retinopathy | curcumin | oxidative stress | Müller cell

ABSTRACT
AIM: To explore the effects and potential mechanisms of curcumin on retinal Müller cell in early diabetic rats.METHODS: Diabetic rats were induced by a single intraperitoneal injection of streptozotocin (STZ). Male Sprague-Dawley (SD) rats were randomly assigned into 4 groups:control group (naïve SD rats administered with a single intraperitoneal injection of citric buffer), diabetic group (STZ-diabetic rats), dimethyl sulfoxide (DMSO) group (diabetic rats intraperitoneally administered with mixture of DMSO and normal saline, once a day) and curcumin group (diabetic rats intraperitoneally administered with curcumin, 80mg/kg, once a day). Three months after diabetes onset, malondialdehyde (MDA, indication of oxidative stress level) and reduced glutathione (GSH) in retina were detected with kits, glial fibrillary acidic protein (GFAP) in retina was revealed by immunohistochemistry and Western blot, and retinal glutamine synthetase (GS) were observed by Western blot.RESULTS: Compared with control group, retinal MDA was increased, and GSH was decreased in diabetic and DMSO groups (P0.05). Furthermore, up-regulation of retinal GFAP and down-regulation of retinal GS were detected in diabetic and DMSO groups, and no alteration could be observed in curcumin group revealed with Western blot. Compared with control group, retinal Müller cells showed significant increase in GFAP immunochemistry staining in diabetic and DMSO groups. Moreover, GFAP-positive staining was decreased in curcumin group compared with diabetic group.CONCLUSION: Curcumin inhibits diabetic retinal oxidative stress, protects Müller cell, and prevents the down-regulation of GS in diabetic retina. Therefore, curcumin has a therapeutic potential in the treatment of diabetic retinopathy (DR).
Affiliate Program      Why do you need a reservation system?