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Protein Electrophoresis and DNA in Herbs Produced from Irradiated Ambrosia maritima Seeds Grown under Soil Salinity and Their Resistance to Insect

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Author(s): O.S. Hussein

Journal: American Journal of Plant Physiology
ISSN 1557-4539

Volume: 7;
Issue: 6;
Start page: 261;
Date: 2012;
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Keywords: Ambrosia maritima | soil salinity | y-rays | insect resistant | DNA | protein electrophoresis

ABSTRACT
Damsisa (Ambrosia maritima L.) is one of the wild plants present in Egypt and different African countries of the Nile Valley. It considered as potential source of molluscicides for treatment of infected sites. In this study, DNA amplifications technique and protein electrophoresis were used for the evaluation of response of Damsisa herbs to gamma rays (γ-rays), soil salinity and their interaction on alleviation of salt stress. This study also examined the effect of herb as bio-resistant for insect infestation in Phaseolus beans. Protein electrophoresis revealed that the number of protein bands separated from plants grown in saline soil not changed either grown from irradiated or un-irradiated seeds except 40 Gray (Gy) dose. Meanwhile, it was observed that mixing Damsisa herb with infested Phaseolus beans reduced insect ability to lays eggs or complete life cycle. Also, it was found that herbs produced from irradiated seeds and grown in normal or in saline soil were more effective in destruction of Callosobruchus maculatus insect and decreased the loss from infested beans.
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