Academic Journals Database
Disseminating quality controlled scientific knowledge

Protein thiols as novel biomarkers in ecotoxicology: A case study of oxidative stress in Mytilus edulis sampled near a former industrial site in Cork Harbour, Ireland

ADD TO MY LIST
 
Author(s): Sara Tedesco | Siti Nur Tahirah Jaafar | Ana Varela Coelho | David Sheehan

Journal: Journal of Integrated OMICS
ISSN 2182-0287

Volume: 2;
Issue: 2;
Start page: 39;
Date: 2012;
Original page

Keywords: Protein thiols | biomarkers | remediation | oxidative stress | proteomics | Mytilus edulis

ABSTRACT
Oxidative stress produces reactive oxygen species which can modify proteins and thiols of cysteines are especially susceptible. Mytilus edulis was sampled from three stations in Cork Harbour, Ireland and from an out-harbour control site in Bantry Bay, Ireland. A variety of traditional biomarkers were benchmarked against thiol oxidation. Lysosomal membrane stability diminished in haemocytes from the three Cork harbour sites, although a stronger effect was observed in two in-harbour stations of environmental concern (Douglas and Haulbowline Island). Catalase and glutathione transferase (GST) activities were decreased in digestive gland extracts of animals from in-harbour sites especially the in-harbour control (Ringaskiddy) showed lower GST than Bantry. Mussels collected at Haulbowline Island showed elevated lipid peroxidation (0.05 > p) compared to the other three stations and decreased levels of protein thiols which is consistent with oxidative stress at this site. Protein profiles for thiol-containing protein sub-proteomes trapped on activated thiol sepharose for each site were obtained by two dimensional electrophoresis and revealed differences between stations. Selected thiol-containing proteins were also identified by in-gel tryptic digestion and mass spectrometry; endoglucase, aginine kinase, creatine kinase 1 and endo-1,4-beta-glucanase. Our findings confirmed that protein thiols are therefore sensitive novel biomarkers to oxidative stress.
Affiliate Program      Why do you need a reservation system?