Academic Journals Database
Disseminating quality controlled scientific knowledge

Purification and Characterization of Fibrinolytic Proteases from Mushroom Volvariela volvaceae Extract.

ADD TO MY LIST
 
Author(s): Dondin Sajuthi | Irma Suparto | Yanti | Willy Praira

Journal: Makara Seri Sains
ISSN 1693-6671

Volume: 14;
Issue: 2;
Start page: 145;
Date: 2010;
Original page

Keywords: fibrinolytic enzyme | purification | Volvariella volvaceae | zymography

ABSTRACT
Purification and Characterization of Fibrinolytic Proteases from Mushroom Volvariela volvaceae Extract. Ediblestraw mushroom (V. volvaceae) has been known used for improvement of blood circulation due to its fibrinolyticcontent. The objective of the study is to purify and characterize fibrinolytic protease from straw mushroom extract.Purification were performed through several steps, i.e. precipitation using ammonium sulphate 75%, dialyzed membran(cut-off 10 kDa), and ion-exchange chromatography using DEAE Sepharose. The active fraction of DEAE-Sepharosecontains two purified protein bands with molecular weight of 12.9 and 15.8 kDa. The active fraction has specificactivity of 0.383 U/mg with 2.7 fold higher purification compared to its crude extract. Both crude and purified enzymeshad optimum activity at temperature of 50 ÂșC and pH 7 in 10 minutes of incubation. Fibrin zymographic profiledemonstrated that the enzyme hydrolyzed fibrin, as well as casein, indicating their potent fibrinolytic activity. Theenzyme was strongly inhibited by phenilmethylsulphonyl fluoride and N-p-tosil-L-lysinchloromethyl keton. Thissuggested that it was a serine protease. In summary, these results showed that crude and purified protease of strawmushroom (V. volvaceae) has fibrinolytic activities that can be applied for alternative thrombolytic therapy.

Tango Rapperswil
Tango Rapperswil

     Affiliate Program