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Purification and characterization of a lectin from leaf pulp of Aloe vera (L.) BURM. F.

Author(s): Manpreet Kaur1, Jatinder Singh 2*, Sukhdev Singh Kamboj2, A.K. Saxena3

Journal: Journal of Pharmacy Research
ISSN 0974-6943

Volume: 4;
Issue: 7;
Start page: 2441;
Date: 2011;
Original page

Keywords: Aloe vera | anti-cancer | anti-insect | Bactrocera cucurbitae | lectin | mucin

A mucin specific lectin was isolated from leaf pulp of Aloe vera (L.) Burm. f., family Aloaceae. The isolation procedure employed affinity chromatography on mucin-linked amino activated silica beads. The purity of the lectin preparation is showh by a single band in SDS-PAGE at pH 8.3 and in native PAGE at pH 4.5. However, multiple bands were obtained in isoelectric focusing and native PAGE at pH 8.3 showing the presence of isolectins. A. vera leaf lectin (AVLL) showeda single peak in gel permeation chromatography on Biogel P-200 and native molecular mass was calculated to be 53 kDa. The hemagglutinating activity of the lectin was not affected upto the temperature of 70°C and in the pH range of 1.5–12.5. The lectin was stable upto 7.0 M concentration of various denaturants tested i.e. urea, thiourea, guanidine hydrochloride and do not require metal ions for its activity. Out of 13 human cancer cell lines tested, AVLL inhibited proliferation of colon cancer cell lines i.e. HCT-15, HT-29 and SW-620 and a lung cancer cell line, HOP-62. This lectin also showed significant inhibitory effect on the development of melon fruit fly, Bactrocera cucurbitae (Coquillett), one of the most important pests of cucurbits. Furthermore, this lectin was found to be virtually non-mitogenic towards human peripheral blood monocytes. However, it was mitogenic towards murine splenocytes with relative mitogenic index approximately double i.e. 232.9, as compared to ConA, a well-known standard mitogen.
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