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Qualitative and quantitative characteristic ofthe population of mesenchymal cells in structural components from conotruncal region of the embryo heart on its early hystogenesis

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Author(s): Dyagovets K.I.

Journal: Морфологія
ISSN 1997-9665

Volume: 6;
Issue: 3;
Start page: 10;
Date: 2012;
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Keywords: conus | truncus | mesenchymal cells | aorticopulmonary septation complex | conotruncal defects

ABSTRACT
Character of the distribution of the mesenchymal’s cellspopulation was analysed during the early hystogenesis of the conotruncal region of the mouse embr yo heart. Conotruncalregion is the predecessor of the proximal part of the aorta and pulmonary trunk, of the aortic ruff and conus arteriosus and semilunar valves. Violation of its transformations leading to the formation of congenital heart disease,which named «conotruncal defects». The material was used embryos mice by the C57BL / 6 line, which covers the period of from 10 to 12.5th day, which corresponded to a 16-21 Teilor stages. Quantify the planar and volumetric distribution of a population of mesenchymal stem cells conotruncus’s pillows and ridges by the original method; on the basis of serial pictures of embryo heart with the use of the computer providing Photoshop Cs5, Amira of for microscopy 5.0, 3ds max 8.0, we created the three-dimensional models of structural components of the conotruncus embryonic mouse heart. During this research noted the phased settlement condensed esenchymal stem fraction first and then tapered sections, given the qualitative and quantitative assessment of the distribution of the conotruncus’s mesenchyme embryonic mouse heart.
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