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QUALITATIVE AND QUANTITATIVE PROFILE OF ALOIN ISOLATED FROM ALOE VERA

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Author(s): Soni Himesh | Sharma Sarvesh | Mishra Kaushelendra | Singhai A.K. | Chaubey Neelesh

Journal: International Research Journal of Pharmacy
ISSN 2230-8407

Volume: 2;
Issue: 9;
Start page: 121;
Date: 2011;
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Keywords: Aloin | A.vera | HPLC | TLC & isolation.

ABSTRACT
A.vera is known to contain well over 100 separate ingredients or constituents between those found in the leaf and those found in the mucilaginous gel inside the leaf. It is also known that some of the ingredients found in the leaf such as Aloin, or the Emodins are recognized as having laxative and antimicrobial properties. Aloin is Folk Traditional Medicine used for Healing of minor cutaneous injuries, such a blisters, abrasions, cuts, burns, bites and scares, protection and care of external skin, such as cleansing, moisturizing, tightening and in mixes as sunscreens and anti-chapping compounds. In the present work, we have investigated the qualitative and quantitative determination of Aloin isolated from the A.vera. Qualitative estimation was carried out by treating the sample with bromine water and thin layer chromatographic (TLC) method. The simultaneous determination of the Aloin was carried out by HPLC technique. HPLC separation was performed on a Cyber Lab C-18 column (250 x 4.0 mm, 5μ) using methanol (A) and 0.34% acetic acid (B) using a isocratic elution as follow: 0–30 min, 40%A–80% A, 60%B–20% B. The flow rate was 1.0 mL/min, and a column temperature of 25°C. The injection volume was 25µl, and UV detection was effected at 297.5 nm.
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