Author(s): Richa Srivastava | Sudhir Shukla | Arvind Soni | Anil Kumar
Journal: Crop Breeding and Applied Biotechnology
ISSN 1518-7853
Volume: 9;
Issue: 2;
Start page: 154;
Date: 2009;
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Keywords: Bougainvillea | RAPD | hybridity | genetic diversity | Principle Component Analysis.
ABSTRACT
The present study deals with authenticating existing knowledge about 21 Bougainvillea cultivars comprisingof 9 hybrids and their parents through RAPD analysis. The 19 degenerate primer sets generated 234 bands from which 158(67.5%) were polymorphic. The UPGMA based dendrogram divided 21 cultivars into two major groups with Jaccard’ssimilarity coefficient ranging from 0.51 to 0.942. Group A had three cultivars namely Trinidad, Formosa and Dr. H. B. Singhin which Dr. H.B. Singh was confirmed as a hybrid of other two cultivars. Group B was sub divided into 8 clusters. Theparentages of 7 out of 8 hybirds have been confirmed based on clusters. The study concluded that the RAPD technique issuitable for confirmation of parent-hybrid relationship.
Journal: Crop Breeding and Applied Biotechnology
ISSN 1518-7853
Volume: 9;
Issue: 2;
Start page: 154;
Date: 2009;
VIEW PDF
DOWNLOAD PDF Original pageKeywords: Bougainvillea | RAPD | hybridity | genetic diversity | Principle Component Analysis.
ABSTRACT
The present study deals with authenticating existing knowledge about 21 Bougainvillea cultivars comprisingof 9 hybrids and their parents through RAPD analysis. The 19 degenerate primer sets generated 234 bands from which 158(67.5%) were polymorphic. The UPGMA based dendrogram divided 21 cultivars into two major groups with Jaccard’ssimilarity coefficient ranging from 0.51 to 0.942. Group A had three cultivars namely Trinidad, Formosa and Dr. H. B. Singhin which Dr. H.B. Singh was confirmed as a hybrid of other two cultivars. Group B was sub divided into 8 clusters. Theparentages of 7 out of 8 hybirds have been confirmed based on clusters. The study concluded that the RAPD technique issuitable for confirmation of parent-hybrid relationship.
