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A Rapid and Efficient Method for the Isolation of Mitochondrial DNA From Wheat Crop

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Author(s): Muhammad Ejaz | Zhang Gaisheng | Zhu Qidi | Zhang Xinbo

Journal: Journal of Agricultural Science
ISSN 1916-9752

Volume: 5;
Issue: 6;
Date: 2013;
Original page

ABSTRACT
The mitochondrial DNA was isolated from wheat (Triticum aestivum) crop in combination of different centrifugation and density gradient ultracentrifugation, DNaseI enzyme treatment, sucrose sedimentation; lysis with SDS and potassium proteinase. Pure mitochondrial DNA was successfully obtained by phenol/chloroform/isoamyl alcohol extraction to remove protein, and digested with RNase phenol/chloroform extraction method. To detect mitochondrial DNA purity, specific primers were designed for nuclear (Beta-actin) and mitochondrial (COXII) gene. Isolated mitochondrial DNA is pure, suitable for PCR and genetic analysis.
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