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Rapid and Facile Purification of Apolipoprotein A-I from Human Plasma Using Thermoresponsive Nanoparticles

Author(s): Martin Lundqvist | Tord Berggård | Erik Hellstrand | Iseult Lynch | Kenneth A. Dawson | Sara Linse | Tommy Cedervall

Journal: Journal of Biomaterials and Nanobiotechnology
ISSN 2158-7027

Volume: 02;
Issue: 03;
Start page: 258;
Date: 2011;
Original page

Keywords: Apolipoprotein A-I | nanoparticles | selective purification

Nanoparticles can be used to purify proteins from plasma. We report here the purification of apolipoprotein A-I (apoA-I) with high specificity from human plasma using copolymeric nanoparticles. We present an optimized protocol using 50:50 NiPAM:BAM copolymer nanoparticles with thermo-responsive properties as an affinity resin. Repeated pelleting and washing of nanoparticle-captured apoA-I is achieved through temperature cycling. The protein is then eluted using urea followed by an ion exchange step for protein concentration and depletion of nanoparticles.
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