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A rapid, non enzymatic method for genomic DNA extraction from whole blood and mammalian tissues

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Author(s): Adnan F. N Al-azawy

Journal: Research Opinions in Animal & Veterinary Sciences
ISSN 2221-1896

Volume: 1;
Issue: 5;
Start page: 279;
Date: 2011;
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Keywords: DNA Extraction | Blood | Mammalian Tissues

ABSTRACT
Although several methods have been exist for DNA extraction from blood or animal tissues samples, traditionally most of these methods consume long time and using expensive chemicals such as proteinase K or toxic organic solvent such as phenol. On the other hand, there is no rapid, simple one method for the extraction of genomic DNA from blood and animal tissues samples in the same time. Since the objective of this study was to development easy modified method for DNA extraction from difference mammalian tissues such as fresh or frozen whole blood, kidney, liver, heart, muscles. The description method have many advantages, reducing the time, using inexpensive materials, no phenol, in addition to small amount of mammalian tissue is required (100-200 mg and 2 ml from whole blood). Genomic DNA was obtained having high molecular weight and good quality, shown by agarose gel electrophoresis and spectrophtometric analysis. These results shown that the modified method is simple, fast, safe, most economical, resulting in a high molecular genomic DNA of good quality from several mammalian tissues and can be used in medical laboratories and research centers.
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