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Rapid Screening of Ammonia Oxidizing Bacteria in the Sewage

Author(s): Cuijuan Zhao | Wenjun Song | Jiping Wei | Bozhi Li

Journal: International Journal of Bioscience, Biochemistry and Bioinformatics
ISSN 2010-3638

Volume: 3;
Issue: 2;
Start page: 93;
Date: 2013;
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Keywords: Sewage | ammonia oxidizing bacteria | rapid screening | identification

The ammonia oxidizing to nitrite is a key step in biological nitrogen removal process in biological wasterwater treatment systems. In order to reduce the concentration of NH4 +-N in the sewage. An novel and rapid method for screening of AOB from the sewage is described in this study, AOB were isolated from Haihe hospital and Jizhuangzi sewage treatment plant. Due to the using of 96-well ELISA plate and nzyme-labelling measuring instrument, the speed of screen was fasted and the time of screen was to be shorten. As a result, 7 efficient bacteria were screened, which ammonia nitrogen removal rate was from 70% to 80%. These results might provide useful and valuable information about the nitritation process, which plays an important role in biological wastewater treatment systems.

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