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The reactivity of Cymbidium hybridum protocorms, sustained on luffa ligno-skeleton in vitrocultures in liquid media

Author(s): Cristian Felix BLIDAR | Dorina CACHITA-COSMA | Gheorghe Emil BANDICI | Adriana PETRUS-VANCEA | Liviu POP | Ildiko SZABO

Journal: Analele Universitatii din Oradea, Fascicula Biologie
ISSN 1224-5119

Volume: TOM XIV;
Start page: 91;
Date: 2007;
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Keywords: Cymbidium (orchid) | growth regulators | “in vitro” | lingo-skeleton | Luffa

Is known the fact that the Cymbidium hybridum orchid protocorms, submersed in liquid culture medium (in hypoxia conditions) himself multiply with higher speed in comparison to aerated condition, on surface of agarized (solid) culture media. Also, is known the fact that the Luffa ligno-skeleton (obtained from ripe pulp of Luffa cilindrica fruits) lends successfully at its usage as wick in supply with nutrients of various plant species inoculs, cultivated “in vitro” on liquid media. In this work, we are presenting the role of Luffa ligno-skeleton in maintaining the Cymbidium hybridum protocorms, cultivated “in vitro”, on culture medium liquid surface (type of Murashige-Skoog, 1962).Behind these experiments, was proved that the ligno-skeleton of Luffa type of bridges for Cymbidium hybridum protocorms, sustained in aerated condition, is more efficient in bearing with those marked on solid culture medium, as in number, as in accumulation of fresh and dry weight, regardless of growth regulators included in media; on liquid culture media containing 1 mg/l AIB or 2 mg/l K as growth regulators, where bearing with the homologue culture variant (solidified media with agar-agar, with same mineral and organic composition), the differences was until 29.3% superior, and bearing with the witness medium (solid – agarized – medium, but without growth regulators) until 30.2% biggest, concerning the number of protocorms.
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