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The reactivity of Cymbidium hybridum L. protocorms vitrocultivated on liquid mediums with fructose as substitute of sucrose, using pylons/hangers of filter paper

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Author(s): Cristian Felix BLIDAR | Gheorghe Emil BANDICI | Ildiko SZABO | Monica MARIAN | Adriana PETRUS-VANCEA | Liviu POP

Journal: Analele Universitatii din Oradea, Fascicula Biologie
ISSN 1224-5119

Volume: TOM XV;
Start page: 13;
Date: 2008;
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Keywords: protocorms | Cymbidium (orchid) | paper-bridge | fructose | “in vitro”

ABSTRACT
In this study, we had proposed to dignify the Cymbidium hybridum protocorms reactions, in a vitroculture regime/condition, on liquid medium MS (1962), maintained on special filter paper bridges (Blidar type [1]), in the conditions of the presence fructose as principal sources of carbon, using various phytohormons. The basic medium culture used by us in these experiments was Murashige – Skoog (1962), without agar. To this, we added different growth regulators, such as: 2,4-D (2 mg/l), or mixture of BA (2 mg/l) with NAA (1 mg/l), or only BA (2 mg/l), or only NAA (1 mg/l). The witness lot was consisted of vitrocultivated protocorms on same liquid medium culture, without growth regulators.At the end of the experimental period, was proved that the stronger multiplication of the protocorms number was registered using medium culture with BA cytochine in a quantity of 2 mg/l, being numerical above 1.7 times more in comparison with the control variant.
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