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Real-time PCR analysis of enteric pathogens from fecal samples of irritable bowel syndrome subjects

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Author(s): Rinttilä Teemu | Lyra Anna | Krogius-Kurikka Lotta | Palva Airi

Journal: Gut Pathogens
ISSN 1757-4749

Volume: 3;
Issue: 1;
Start page: 6;
Date: 2011;
Original page

ABSTRACT
Abstract Background Growing amount of scientific evidence suggests that microbes are involved in the pathophysiology of irritable bowel syndrome (IBS). The predominant fecal microbiota composition of IBS subjects has been widely studied with DNA-based techniques but less research has been focused on the intestinal pathogens in this disorder. Here, we optimized a highly sensitive panel of 12 quantitative real-time PCR (qPCR) assays to shed light on the putative presence of intestinal pathogens in IBS sufferers. The panel was used to screen fecal samples from 96 IBS subjects and 23 healthy controls. Results Fifteen IBS samples (17%) tested positive for Staphylococcus aureus with a thermonuclease (nuc) gene-targeting qPCR assay, whereas none of the healthy controls were positive for S. aureus (p
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