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Reduced expression of cenp-e in human hepatocellular carcinoma

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Author(s): Liu Zijie | Ling Kang | Wu Xia | Cao Ju | Liu Bin | Li Suyan | Si Qiong | Cai Yan | Yan Chen | Zhang Yan | Weng Yaguang

Journal: Journal of Experimental & Clinical Cancer Research
ISSN 1756-9966

Volume: 28;
Issue: 1;
Start page: 156;
Date: 2009;
Original page

ABSTRACT
Abstract Background CENP-E, one of spindle checkpoint proteins, plays a crucial role in the function of spindle checkpoint. Once CENP-E expression was interrupted, the chromosomes can not separate procedurally, and may result in aneuploidy which is a hallmark of most solid cancers, such as hepatocellular carcinoma (HCC). We investigate the expression of CENP-E in human hepatocellular carcinoma,. and analyze the effect of low CENP-E expression on chromosome separation in normal liver cell line (LO2). Methods We determined its levels in HCC and para-cancerous tissues, human hepatocellular carcinoma-derived cell line (HepG2) and LO2 cell line using real time quantitative PCR (QPCR) and Western blot. Further to know whether reduction in CENP-E expression impairs chromosomes separation in LO2 cells. we knocked down CENP-E using shRNA expressing vector and then count the aneuploid in LO2 cells using chromosomal counts assay. Results We found that both CENP-E mRNA and protein levels were significantly reduced in HCC tissues and HepG2 cells compared with para-cancerous tissues and LO2 cells, respectively. A significantly-increased proportion of aneuploid in these down-knocked LO2 cells compared with those treated with control shRNA vector. Conclusions Together with other results, these results reveal that CENP-E expression was reduced in human HCC tissue, and low CENP-E expression result in aneuploidy in LO2 cells.

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