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Research: STUDY THE POSSIBLE HEPATOPROTECTIVE EFFECT OF DIFFERENT DOSES OF Ammi majus SEEDS' EXTRACT AGAINST CCl4 INDUCED LIVER DAMAGE IN RATS

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Author(s): Shihab Hattab Mutlag | Dawser K Ismael | Nada N Al-Shawi*

Journal: Pharmacie Globale : International Journal of Comprehensive Pharmacy
ISSN 0976-8157

Volume: 02;
Issue: 09;
Start page: 1;
Date: 2011;
Original page

Keywords: Ammi majus | CCl4 induced-hepatotoxcity | hyperbilirubemia.

ABSTRACT
Liver is considered as the major organ responsible for conducting various metabolic processes and according to it’s highly exposed to toxic effect of different xenobiotics predisposing to many types of diseases and disorders. The role of plant with antioxidant activity in the treatment and prevention of chemical-induced liver damage was extensively studies. Ammi majus show antioxidant effect their use in diabetic nephropathy and myocardial injury due to the presence of different active constituent such as quercetine, marmesinin, kempefrol and other compounds that inhibit cytochrome p450 such as xanthotoxin bergapten, imperatorin and isoimpinellin. Accordingly, this study was designed to evaluate the hepatoprotective effect of the aqueous solution of ethanolic extract of the Ammi majus against carbon tetrachloride (CCl4) induced liver damage in rats. Eighty adult rats of both sex divided into four groups allocated as follows: Group I- (negative control), rats received D.W 2ml/kg for 14 days. Group II- rats treated with single oral daily dose Ammi majus seeds extract 16mg/rat/day alone for 14 days. The animals of groups I and II were sacrificed by anesthetic ether on the day 15. Group III- (positive control) rats received single oral daily dose of 2ml/kg/day D.W. for 14 days and at the day 15, the animals received single oral dose of CCl4, the animals were sacrificed by anesthetic ether 24 hr after CCl4 administration. Groups IV (A, B, C, D and E) received either (1mg or 2mg or 4mg or 8mg or 16mg/rat/day), respectively for 14 days of Ammi majus ethanolic extract then at the day 15 they received single dose of CCl4 then sacrificed after 24 hours after CCl4 administration. Rats' livers were obtained for preparation of tissue homogenate to evaluate MDA & GSH in the hepatic homogenate as indicator of lipid peroxidation. Blood samples were collected by intra-cardiac puncture, and utilized for evaluating serum enzymes activities manifested by aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) in addition for assessing total serum Bilirubin (TSB). Analysis of data revealed significant amelioration of oxidative stress in rats pre-treated with different doses of Ammi majus extract (4mg,8mg and 16mg/rat/day for 14days) compared to group III of animals intoxicated by CCl4 as evidenced by lowering MDA contents and elevation of GSH levels in liver tissue homogenate but the levels still significantly different compared to controls. Elevation of serum activities of ALT, AST and ALP, in addition to TSB levels as a results of treatment with toxic dose of CCl4 was significantly reduced by pre-treatment with different doses of Ammi majus extract but the levels still significant different from control. Ammi majus extract also attenuated hyperbilirubemia caused by CCl4 intoxication. From the data obtained from this work, we can conclude that the extract of Ammi majus showed protective effect against CCl4 induced-hepatotoxcity.
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