Author(s): Janusz Piotr Sikora | Danuta Chlebna-Sokol | Ewa Andrzejewska | Slawomir Chrul | Wojciech Kuzanski | Anna Sikora
Journal: Przegląd Pediatryczny
ISSN 0137-723X
Volume: 37;
Issue: 1;
Start page: 95;
Date: 2007;
Original page
ABSTRACT
The aim of the study was the evaluate the intensity of respiratory burst of peripheral blood polymorphonuclear leukocytes (PMNLs), as we ll as the expression of p55 and p75 TNF-a receptors on those cell surfaces in severely burned children. Material and methods: The study included 17 patients (11 boys and 6 girls) with severe burns, median age - 18 months, with total body surface area (TBSA) varying from 10 to 25%, qualified as mixed burns (IIa/IIb/III). The control group included 21 age matched children without the signs of inflammatory process. Immunological indices mentioned above were evaluated with the BURSTTEST reagent kit and monoclonal antibodies conjugated with fluorescein or phycoerythrin using flow cytometry. Results: We observed a significant increase in the respiratory burst of PMNLs compared with the initial values in severely burned children after the termination of therapy. Moreover, significant decrease of p55 and p75 TNF-a receptor expressions on the surface of those cells compared with the initial values after therapy termination was found. Conclusion: Evaluation of the presented immunological indices indicates the role of TNF-a and reactive oxygen species in SIRS (systemic inflammatory response syndrome) pathogenesis in the course of severe burns in children, which may support diagnostic and prognosis methods.
Journal: Przegląd Pediatryczny
ISSN 0137-723X
Volume: 37;
Issue: 1;
Start page: 95;
Date: 2007;
Original page
ABSTRACT
The aim of the study was the evaluate the intensity of respiratory burst of peripheral blood polymorphonuclear leukocytes (PMNLs), as we ll as the expression of p55 and p75 TNF-a receptors on those cell surfaces in severely burned children. Material and methods: The study included 17 patients (11 boys and 6 girls) with severe burns, median age - 18 months, with total body surface area (TBSA) varying from 10 to 25%, qualified as mixed burns (IIa/IIb/III). The control group included 21 age matched children without the signs of inflammatory process. Immunological indices mentioned above were evaluated with the BURSTTEST reagent kit and monoclonal antibodies conjugated with fluorescein or phycoerythrin using flow cytometry. Results: We observed a significant increase in the respiratory burst of PMNLs compared with the initial values in severely burned children after the termination of therapy. Moreover, significant decrease of p55 and p75 TNF-a receptor expressions on the surface of those cells compared with the initial values after therapy termination was found. Conclusion: Evaluation of the presented immunological indices indicates the role of TNF-a and reactive oxygen species in SIRS (systemic inflammatory response syndrome) pathogenesis in the course of severe burns in children, which may support diagnostic and prognosis methods.
