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The results in the field of the in vitro conservation of the cultivars when using classic and modern conservation methods

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Author(s): Anca BACIU | Adriana PETRUS-VANCEA | Rodica ZEHAN | Luiza MIKE | Mihaela PRODAN

Journal: Analele Universitatii din Oradea, Fascicula Biologie
ISSN 1224-5119

Volume: TOM XIV;
Start page: 23;
Date: 2007;
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Keywords: methods | conservation | in vitro | in situ

ABSTRACT
By means of the in situ conservation the vegetal material is exposed to genetic modifications as a consequence of the natural or artificial selecting. The ex situ conservation, by the use of traditional techniques, allows the keeping of seeds, plants or different plant organs elsewhere than in their originating places for a long period of time and in good conditions. The ex situ conservation may be in gene banks, in botanical gardens or in sanctuaries. The costs for such conservation are very high and it also runs the risk of losing the material because of pests, diseases or the unfavorable climatic conditions. The in vitro conservation eliminates all these disadvantages allowing the conservation of the vegetal material for a very long period of time. On the other hand by using this method the rapid multiplication of the stored material is possible. The in vitro conservation techniques can facilitate the application of some genetic handling procedures such as the putting at the disposal of a simple way to stock the experimental material in the form of in vitro cultures. The new conservation techniques give a new impetus to all in vitro culture conservation methods. The inoculums cultivated on aseptic media can be preserved in a certain morpho – physiological stage and their regenerative activity can start anew by using variety of types of techniques such as frost conservation (The frosting or the keeping of the inoculums at positive or low temperatures), cryopreservation; the conservation in conditions of inoculums slow growth; the inoculums dehydration ( up to the surviving limit); the growths of the inoculums at low air pressure or in hypoxia; The goal of the present study is to find out appropriate conservation methods of the performing genotypes at different culture species.
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